af488 nt labelling kit (Jena Bioscience)
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af488 nt labelling kit
Af488 Nt Labelling Kit, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/af488+nt+labeling+kit/AF488+NT+Labeling+Kit/pmc10913579-72-27-33
Average 90 stars, based on 5 article reviews
Af488 Nt Labelling Kit, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/af488+nt+labeling+kit/AF488+NT+Labeling+Kit/pmc10913579-72-27-33
Average 90 stars, based on 5 article reviews
af488 nt labelling kit - by Bioz Stars,
2026-10
90/100 stars
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In Situ:Article Title: Meiotic instability and irregular chromosome pairing underpin heat-induced infertility in bread wheat carrying the Rht-B1b or Rht-D1b Green Revolution genes. Article Snippet: .. In situ hybridisation The wheat centromeric retrotransposon (CRW; Li et al., 2013) and the universal plant telomeric repeat (TRS; Schwarzacher & Heslop-Harrison, 1991) were amplified by PCR and labelled by nick-translation ( Hybridization:Article Title: Meiotic instability and irregular chromosome pairing underpin heat-induced infertility in bread wheat carrying the Rht-B1b or Rht-D1b Green Revolution genes. Article Snippet: .. In situ hybridisation The wheat centromeric retrotransposon (CRW; Li et al., 2013) and the universal plant telomeric repeat (TRS; Schwarzacher & Heslop-Harrison, 1991) were amplified by PCR and labelled by nick-translation ( Amplification:Article Title: Meiotic instability and irregular chromosome pairing underpin heat-induced infertility in bread wheat carrying the Rht-B1b or Rht-D1b Green Revolution genes. Article Snippet: .. In situ hybridisation The wheat centromeric retrotransposon (CRW; Li et al., 2013) and the universal plant telomeric repeat (TRS; Schwarzacher & Heslop-Harrison, 1991) were amplified by PCR and labelled by nick-translation ( Article Title: Rapid in-solution preparation of somatic and meiotic plant cell nuclei for high-quality 3D immunoFISH and immunoFISH-GISH. Article Snippet: .. Amplified DNA sequences (FISH) or total barley DNA (GISH) were directly labelled by nick-translation ( Article Title: Plasticity of parental CENH3 incorporation into the centromeres in wheat × barley F1 hybrids Article Snippet: To obtain the FISH probe, the DNA sequence covering the barley 5S rDNA coding and noncoding flanking regions ( ) was amplified by PCR and labelled with an AF488 NT Labeling Kit (Jena Bioscience, PP-305L-AF488). .. To obtain the FISH probe, the DNA sequence covering the barley 5S rDNA coding and noncoding flanking regions ( ) was amplified by PCR and labelled with an Polymerase Chain Reaction:Article Title: Meiotic instability and irregular chromosome pairing underpin heat-induced infertility in bread wheat carrying the Rht-B1b or Rht-D1b Green Revolution genes. Article Snippet: .. In situ hybridisation The wheat centromeric retrotransposon (CRW; Li et al., 2013) and the universal plant telomeric repeat (TRS; Schwarzacher & Heslop-Harrison, 1991) were amplified by PCR and labelled by nick-translation ( Article Title: Plasticity of parental CENH3 incorporation into the centromeres in wheat × barley F1 hybrids Article Snippet: To obtain the FISH probe, the DNA sequence covering the barley 5S rDNA coding and noncoding flanking regions ( ) was amplified by PCR and labelled with an AF488 NT Labeling Kit (Jena Bioscience, PP-305L-AF488). .. To obtain the FISH probe, the DNA sequence covering the barley 5S rDNA coding and noncoding flanking regions ( ) was amplified by PCR and labelled with an Labeling:Article Title: Meiotic instability and irregular chromosome pairing underpin heat-induced infertility in bread wheat carrying the Rht-B1b or Rht-D1b Green Revolution genes. Article Snippet: .. In situ hybridisation The wheat centromeric retrotransposon (CRW; Li et al., 2013) and the universal plant telomeric repeat (TRS; Schwarzacher & Heslop-Harrison, 1991) were amplified by PCR and labelled by nick-translation ( Article Title: Rapid in-solution preparation of somatic and meiotic plant cell nuclei for high-quality 3D immunoFISH and immunoFISH-GISH Article Snippet: FISH probes were labelled in a PCR machine at 15°C for 60 min. For the GISH probes, heat-fragmented (6 min, pressure cooker) total DNA was labelled at 15°C for 100 min. Pretreatments Cell permeabilisation was ensured by pipetting 50 μL of 1X PBS containing 0.3% (w/v) CHAPS zwitterionic surfactant (Sigma-Aldrich, C3023), 0.3% (v/v) Triton X-100, and 0.3% (v/v) Igepal CA-630 on the slides and covering them with a plastic coverslip. .. Ampli ed DNA sequences (FISH) or total barley DNA (GISH) were directly labelled by nick-translation ( Article Title: Rapid in-solution preparation of somatic and meiotic plant cell nuclei for high-quality 3D immunoFISH and immunoFISH-GISH. Article Snippet: .. Amplified DNA sequences (FISH) or total barley DNA (GISH) were directly labelled by nick-translation ( Article Title: Plasticity of parental CENH3 incorporation into the centromeres in wheat × barley F1 hybrids Article Snippet: To obtain the FISH probe, the DNA sequence covering the barley 5S rDNA coding and noncoding flanking regions ( ) was amplified by PCR and labelled with an AF488 NT Labeling Kit (Jena Bioscience, PP-305L-AF488). .. To obtain the FISH probe, the DNA sequence covering the barley 5S rDNA coding and noncoding flanking regions ( ) was amplified by PCR and labelled with an Article Title: A Multigraph-Based Representation of Hi-C Data. Article Snippet: To localise active centromeres, immunolabelling was carried out with a rabbit anti-grass CENH3 antibody [35] detected by a goat anti-rabbit abberior Star Red (STRED-1002-500UG, Abberior Genes 2022, 13, 2189 6 of 15 Instruments, Göttingen, Germany) secondary antibody. .. Simultaneous in-situ hybridization included Nick-translation labelled genomic DNA from barley (AF594 NT Labelling Kit, PP305L-AF594, Jena Bioscience, Jena, Germany) and the TRS-probe ( Fluorescence In Situ Hybridization:Article Title: Rapid in-solution preparation of somatic and meiotic plant cell nuclei for high-quality 3D immunoFISH and immunoFISH-GISH Article Snippet: FISH probes were labelled in a PCR machine at 15°C for 60 min. For the GISH probes, heat-fragmented (6 min, pressure cooker) total DNA was labelled at 15°C for 100 min. Pretreatments Cell permeabilisation was ensured by pipetting 50 μL of 1X PBS containing 0.3% (w/v) CHAPS zwitterionic surfactant (Sigma-Aldrich, C3023), 0.3% (v/v) Triton X-100, and 0.3% (v/v) Igepal CA-630 on the slides and covering them with a plastic coverslip. .. Ampli ed DNA sequences (FISH) or total barley DNA (GISH) were directly labelled by nick-translation ( Article Title: Rapid in-solution preparation of somatic and meiotic plant cell nuclei for high-quality 3D immunoFISH and immunoFISH-GISH. Article Snippet: .. Amplified DNA sequences (FISH) or total barley DNA (GISH) were directly labelled by nick-translation ( Article Title: Plasticity of parental CENH3 incorporation into the centromeres in wheat × barley F1 hybrids Article Snippet: To obtain the FISH probe, the DNA sequence covering the barley 5S rDNA coding and noncoding flanking regions ( ) was amplified by PCR and labelled with an AF488 NT Labeling Kit (Jena Bioscience, PP-305L-AF488). .. To obtain the FISH probe, the DNA sequence covering the barley 5S rDNA coding and noncoding flanking regions ( ) was amplified by PCR and labelled with an other:Article Title: Rapid in-solution preparation of somatic and meiotic plant cell nuclei for high-quality 3D immunoFISH and immunoFISH-GISH Article Snippet: Cell permeabilisation was ensured by pipetting 50 μL of 1× PBS containing 0.3% (w/v) CHAPS zwitterionic surfactant (Sigma-Aldrich, C3023), 0.3% (v/v) Triton X-100, and 0.3% (v/v) Igepal CA-630 on the slides and covering them with a plastic coverslip. Sequencing:Article Title: Plasticity of parental CENH3 incorporation into the centromeres in wheat × barley F1 hybrids Article Snippet: To obtain the FISH probe, the DNA sequence covering the barley 5S rDNA coding and noncoding flanking regions ( ) was amplified by PCR and labelled with an AF488 NT Labeling Kit (Jena Bioscience, PP-305L-AF488). .. To obtain the FISH probe, the DNA sequence covering the barley 5S rDNA coding and noncoding flanking regions ( ) was amplified by PCR and labelled with an In Situ Hybridization:Article Title: A Multigraph-Based Representation of Hi-C Data. Article Snippet: To localise active centromeres, immunolabelling was carried out with a rabbit anti-grass CENH3 antibody [35] detected by a goat anti-rabbit abberior Star Red (STRED-1002-500UG, Abberior Genes 2022, 13, 2189 6 of 15 Instruments, Göttingen, Germany) secondary antibody. .. Simultaneous in-situ hybridization included Nick-translation labelled genomic DNA from barley (AF594 NT Labelling Kit, PP305L-AF594, Jena Bioscience, Jena, Germany) and the TRS-probe ( |